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Environ Sci Technol 54(4):22442256

These data suggest that Mecp2 -mediated let-7f upregulation leads to IGF-1 depletion in Rett syndrome, implicating let-7f as a potential therapeutic target for Rett syndrome

Cell suspensions were lysed by freeze-thawing, and PV VLPs were purified from clarified supernatants by concentration through a 30% sucrose cushion followed by a 1545% sucrose gradient in 1 Dulbeccos phosphate-buffered saline (DPBS, Gibco), 20 mM EDTA, pH 7.0 (DPBS-EDTA) 15

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The extra fluid volume can stress an already compromised cardiovascular system

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